Cisplatin cck8

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August undefined, 2024

http://www.rx-8.com/chanpinzhongxin/203370.html WebApr 9, 2024 · Then cells were treated with cisplatin as indicated concentrations. 24 or 48 h later, 10 μL CCK8 reagent (HY-K0301, MedChemExpress, USA) was added into each well for another 2 h. Then the absorbance was measured at 490 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader (Bio Tek).

[miR-29b Reduces Cisplatin Resistance of Gastric Cancer Cell by ...

WebMay 27, 2024 · Uncovering the role of p62 in the development of cisplatin resistance in ovarian cancer. ... (IHC) and its proliferation and invasion effects via CCK8 and transwell assays. Additionally, expression of cyclin D1, MMP2, p‐ERK, and EMT‐related proteins was measured by western blotting. We found that SIX1 had significantly higher expression in ... Web目的探讨姜黄挥发油(TVO)联合顺铂对人皮肤鳞状细胞癌(鳞癌)A431细胞增殖、凋亡的影响及机制.方法取对数生长期A431细胞,用5、10、20、40、80 mg/L TVO处理,对照组用含有1％二甲基亚砜(DMSO)的DMEM高糖培养基处理,24 h后,CCK8法检测细胞增殖情况.另取部分A431细胞,分为4组,分别用含1％ DMSO的DMEM高糖培养基(对照 ... lords prayer in german

HOXB7 mediates cisplatin resistance in esophageal squamous cell ...

WebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the … WebSep 30, 2024 · These cell strains were used to determine the effect of HOXB7 on cell viability, and cell apoptosis after exposure to cisplatin. CCK8 assay showed that treatment for 24 hours with cisplatin resulted in decreased IC50 of HOXB7‐knockdown cells compared with control cells (Fig (Fig3a). 3 a). This means that knockdown of HOXB7 … WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, ... Cell counting kit-8 assay. Cell viability was measured using the CCK-8 (SAB Biotech., College Park, MD, USA) assay. Cells were cultured in 96-well plates at 3 × 10 3 cells/well for 12 h. Following treatment for 48 h, the CCK-8 reagent was ... lords pool

Celastrus Orbiculatus Extract Potentiates the Sensitivity of Cisplatin …

cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐 - 西安瑞禧生物技 …

WebCCK8 assays were also utilized to detect IC50 values for cisplatin and 5-Fu. Results: The expression of circNRIP1 was significantly increased in the serum of chemoresistant NPC patients. At a functional level, we determined that circNRIP1 is able to sequester miR-515-5p, thereby inhibiting its ability to post-transcriptionally suppress IL-25 ... WebSep 30, 2024 · Cisplatin was the first platinum-based agent shown to have anti-tumor properties. 2 Cisplatin-based combinations remain first-line therapy for a number of solid tumors including lung, ovarian, and esophageal cancer. 3 It induces DNA damage through DNA cross-linking, thus interfering with the fundamental processes of DNA replication … horizon nj health join the networkWebSep 18, 2024 · National Center for Biotechnology Information lords prayer leonard warren

"WebJan 1, 2024 · New therapy targets are urgently needed to reverse DDP resistance. Methods: The protein levels of these molecules were analyzed by Western blotting and immunofluorescent staining. Cell proliferation was detected by CCK8 and MTT assays. Cell migration and invasion were analyzed by wound healing assay and Transwell assays. " - Cisplatin cck8

Cisplatin cck8

Cisplatin-resistant HeLa cells are resistant to apoptosis via p53 ...

WebMar 15, 2024 · In vivo experiment showed that pretreatment with anemoside B4 effectively adjusted body weight and kidney index, and reduced cisplatin-elevated blood urea nitrogen (BUN) and creatinine (CREA) levels, as well as ameliorated the histopathological damage. WebJun 11, 2024 · Cck8 colorimetric assay was used to detect the sensitivity of cells to anticancer drugs, cell viability and proliferation in response to drug treatments in vitro. They were also used to determine the concentration of the drug that inhibited cell growth by 50% (IC 50) after 24 h of treatment.

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WebAug 8, 2024 · a Before and after treatment with the supernatants of Tregs, cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM) was analyzed by the CCK8 assay. b With different concentrations of Treg supernatants (supernatant frequency of Treg/tumor cell = 1:1, 1:2 and 1:3), cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM ... WebOct 22, 2024 · Cell viability was analyzed by the Cell Counting Kit-8 (CCK8). The relationship of Nrf2 and CD99 was assessed by dual-luciferase reporter gene assay and chromatin immunoprecipitation (ChIP). Bioinformatics analysis was performed to search for the downstream gene of CD99.

WebCCK8 assays Cell viability was measured by cell counting kit-8 (CCK8). The cells were seeded into 96-well plates with 10 μL of CCK8 reagent in every well, and there were 4 × 104 cells in each well, all incubated at 37°C for 1 h. The optical density (OD) values were measured at 450-nm wavelength by Multiskan FC (Thermo Fisher Scientific, Inc.). WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of...

WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ... WebNational Center for Biotechnology Information

WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was …

WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … lords prayer bracelets for womenWebFeb 16, 2024 · Generic name: cisplatin [ sis-PLA-tin ] Brand names: Platinol, Platinol-AQ, Platinol (Restricted Access) Dosage forms: intravenous powder for injection (50 mg), … lords prayer on a pin headWebObjective: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin. Methods: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to … lords procedureWeb瑞禧生物提供科研级cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购cisplatin-PEG-NPC顺铂-聚乙二醇-硝基 ... lords prayer in kiswahili lords prayer our father pdfWebJul 4, 2024 · To investigate whether miR‐320a regulates laryngeal carcinoma cell cisplatin resistance, miR‐320a overexpression HEp‐2 cells were treated with various concentration of cisplatin. CCK8 assays demonstrated that miR‐320a overexpression significantly inhibited HEp‐2 cell viability (Figure 4 E). lords prayer in icelandicWebWe established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827). The EGFR-related signals were examined by Western blotting. MTT assay and the trypan blue exclusion method were used for the in vitro study, while tumor size and the SUV of the 18FDG-PET scans were … lords press office